Testonic® Pesticide Residue Test Kit is a practical, colour-change-based test kit designed for rapid preliminary screening of organophosphate and carbamate pesticide residues that may be present on fruit and vegetable surfaces.
Suitable for apples, grapes, strawberries, tomatoes, cucumbers, peppers and other fruits and vegetables, the test requires no measuring instrument. It works by preparing a surface sample with the extraction solution and evaluating the colour development on the test card.
Why Is Pesticide Residue Screening Important?
Crop protection products used in agriculture may leave residues on fruit and vegetable surfaces, depending on application conditions. Rapid pesticide screening helps consumers, producers and food businesses perform preliminary checks on potentially contaminated samples.
How to Perform the Pesticide Test
Prepare a sample collected from the fruit or vegetable surface using the extraction solution according to the instructions.
Apply the prepared sample extract to the test card and fold the card as instructed.
After the specified reaction time, compare the developed colour with the control to evaluate the result.
Kit Contents
- 20 single-use pesticide test cards
- Extraction solution
- Sample transfer pipette
- Instructions for use
*The card reaction and result evaluation take approximately 5 minutes. Sample preparation may increase the total procedure time. The kit provides qualitative preliminary screening for organophosphate and carbamate pesticides.
Testonic® Pesticide Residue Test Kit
Testonic® Pesticide Residue Test Kit is a practical, colour-change-based test kit designed for rapid preliminary screening of organophosphate and carbamate pesticide residues that may be present on fruit and vegetable surfaces.
Suitable for apples, grapes, strawberries, tomatoes, cucumbers, peppers, leafy vegetables and other appropriate products, the kit enables preliminary sample assessment without laboratory instruments through enzyme inhibition-based test cards.
Analytical Principle
The test is based on the inhibition of the acetylcholinesterase (AChE) enzyme. Organophosphate and carbamate pesticides may reduce or inhibit acetylcholinesterase activity.
The enzyme and colour-development system on the test card produces a characteristic blue colour under suitable reaction conditions. Compounds that inhibit the enzyme may reduce or prevent colour development.
The colour developed on the sample card is compared with an appropriate control card to obtain a qualitative preliminary screening result.
Normal enzyme activity → Blue colour development
Partial enzyme inhibition → Weaker colour development
Strong enzyme inhibition → Very weak or absent colour development
The colour result does not independently identify or quantify a specific pesticide. Other enzyme-inhibiting substances present in food samples may also affect the reaction.
Importance and Applications of Pesticide Residue Screening
Crop protection products used in agriculture may leave residues on fruit and vegetable surfaces depending on application rates, timing, pre-harvest intervals and environmental conditions.
Rapid pesticide screening provides a practical preliminary assessment, particularly when many products need to be checked, helping identify suspicious samples for further laboratory analysis where necessary.
- Home users: Performing preliminary checks on the surfaces of purchased fruits and vegetables.
- Agricultural producers: Screening selected products before harvest and during production.
- Greengrocers and supermarkets: Conducting preliminary checks on fresh produce received from suppliers.
- Food processing facilities: Screening selected incoming raw material samples.
- Restaurants and catering businesses: Performing preliminary checks on fresh produce supplies.
- Wholesale markets and storage facilities: Conducting rapid screening during product reception and batch checks.
How to Perform the Pesticide Test
Collect a representative sample from the outer surface of the fruit or vegetable. Prepare and shake it with the specified amount of extraction solution according to the instructions.
Apply the specified amount of prepared extract to the white reaction area of the test card. After the pre-reaction period, fold the card along the marked line.
Open the card after the specified reaction time. Compare the developed colour with an appropriate control card.
Note: Sample quantity, extraction solution volume, pre-reaction time and post-folding incubation time must follow the instructions for use. Sample preparation may increase the total procedure time.
Technical Specifications
| Specification | Description |
|---|---|
| Brand | Testonic® |
| Product Name | Pesticide Residue Test Kit |
| Test Principle | Acetylcholinesterase (AChE) enzyme inhibition |
| Target Pesticide Groups | Organophosphates and carbamates |
| Sample Type | Suitable extracts prepared from fruit and vegetable surfaces |
| Analytical Method | Enzyme inhibition / colour comparison |
| Test Capacity | 20 single-use test cards |
| Reaction and Reading Time | Approximately 5 minutes, excluding sample preparation |
| Result Type | Qualitative screening: negative, suspicious or positive |
| Result Evaluation | Visual colour comparison with a control |
| Additional Instruments | Not required |
| Kit Contents | 20 test cards, extraction solution, transfer pipette and instructions for use |
Interpretation of Results
The colour developed on the test card is evaluated by comparison with an appropriate control card. A valid control reaction should produce the expected blue colour development.
Blue colour similar to control
No significant enzyme inhibition is observed.
Weak blue colour
Partial inhibition or analytical interference may be present.
Absence of colour development
Strong enzyme inhibition is observed.
A negative result indicates that no significant enzyme inhibition was observed under the test conditions. It does not prove that the sample is completely free from all pesticides.
A suspicious or positive result indicates reduced enzyme activity. It does not conclusively identify or quantify a specific pesticide. Where necessary, confirmatory laboratory methods such as LC-MS/MS or GC-MS/MS should be used.
Analytical Interferences and Measurement Limitations
Acetylcholinesterase activity may be influenced not only by pesticides but also by the natural chemical characteristics of the sample. Results from certain fruits and vegetables therefore require careful interpretation.
- Acidity and pH: Extracts from acidic fruits such as lemons, oranges, mandarins and grapefruits may affect enzyme activity and colour development.
- Natural plant compounds: Phenolic compounds, sulfur-containing substances and certain plant metabolites may interfere with enzyme activity.
- Strong pigments: Dark-coloured fruits and vegetables may interfere with visual interpretation of the reaction colour.
- Sample preparation: Differences in extraction solution volume, sample ratio or incubation times may affect test repeatability.
- Differences between pesticides: Individual organophosphate and carbamate pesticides do not inhibit the enzyme equally. Test sensitivity may vary depending on the active substance.
- Measurement scope: The test does not cover all pesticide groups and does not independently identify or quantify specific pesticide compounds.
Unexpected results, particularly from acidic, strongly pigmented or naturally enzyme-inhibiting samples, should be repeated and confirmed by laboratory analysis when necessary.
Related Food Safety and Analytical Parameters
| Parameter | What Does It Indicate? |
|---|---|
| AChE Inhibition | Indicates reduced enzyme activity. This is the primary analytical principle of the kit. |
| Organophosphates | A chemical group that includes pesticides capable of inhibiting acetylcholinesterase. |
| Carbamates | A pesticide group whose members may include acetylcholinesterase inhibitors. |
| LC-MS/MS and GC-MS/MS | Laboratory techniques used with appropriate methods to confirm the identity and concentration of specific pesticides. |
Important Usage Notes
- Read the instructions before collecting and testing samples.
- Do not change the specified sample amount or extraction solution ratio.
- Apply the extract only to the designated reaction area of the test card.
- Do not touch the reaction areas with your fingers.
- Fold the card along the marked line to ensure proper contact between reaction areas.
- Follow the specified reaction and reading times.
- Compare results with an appropriate control.
- Use each test card only once.
- Confirm suspicious or positive results through appropriate laboratory analysis.
- Do not treat the test result alone as proof of compliance with legal pesticide residue limits.
Frequently Asked Questions
It provides rapid preliminary screening for organophosphate and carbamate pesticides in samples prepared from fruit and vegetable surfaces.
The kit can be used with apples, grapes, strawberries, tomatoes, cucumbers, peppers and other suitable produce. Natural sample composition may affect test results.
No. It primarily screens for organophosphate and carbamate pesticides capable of inhibiting acetylcholinesterase.
A positive result indicates significant enzyme inhibition. Laboratory confirmation is required to establish whether a particular pesticide is responsible.
No. A negative result indicates that significant enzyme inhibition was not observed under the test conditions. It does not exclude other pesticides.
Yes. Acidic samples and certain natural plant compounds may affect enzyme activity. Results from these samples should be interpreted carefully.
No. The kit provides qualitative preliminary screening. Appropriate laboratory methods are required to identify and quantify specific pesticides.
The control helps verify that the expected test reaction occurs. Sample colour development is interpreted by comparison with the control.
The kit contains 20 single-use test cards.
No. The kit is designed for practical field use with an extraction solution and colour-change-based test cards.
Technical Documents
| Document / Access | Language | ||
|---|---|---|---|
|
Safety Data Sheet (SDS)
Members Only
AChE Sample Equilibration SolutionPesticide Test Kit - 291- | Barkod: 8680131852918 |
- | ||
|
Safety Data Sheet (SDS)
Members Only
Test Card - 291- | Barkod: 8680131852918 |
- |
Technical Support and Product Consulting
Customer Questions & Expert Answers
Can I compare fruits and vegetables before and after washing?
Is it easy to use at home?
What is the purpose of the AChE Sample Balancing Solution?
Is an instrument required to read the result?
How long does it take to obtain a test result?
Can it be used before sending samples to a laboratory?
Can it be used for incoming raw material inspection and comparison of different suppliers or batches?
What benefits does the Pesticide Test Kit provide to our business?
Which pesticides and commercial crop-protection products can the test screen for?
What is AChE inhibition and how does the test detect pesticide residues?
You can ask us about the product.
