Classic hot staining method for the microscopic detection of acid-fast bacteria
The Ziehl–Neelsen Staining Kit is a ready-to-use, three-component system designed for the detection of acid-fast bacilli (AFB), especially Mycobacterium tuberculosis, under light microscopy. Based on the traditional hot staining method, the kit includes Carbol Fuchsin, Acid Alcohol, and an alkaline Methylene Blue counterstain.
Enhanced with Tween 80, methanol, and pH 10.7 buffering, it delivers superior staining contrast, smear uniformity, and long-term stability. Ideal for clinical microbiology labs, TB screening programs, and diagnostic or educational use.
Applications:
-
Identification of Mycobacterium and other acid-fast organisms
-
Clinical microbiology and pathology labs
-
Public health and infectious disease screening
-
Laboratory technician education
-
Standard hot Ziehl–Neelsen staining
Kit Contents (3 × 1000 mL):
|
Solution |
Description |
Volume |
|---|---|---|
|
A |
Carbol Fuchsin (Basic Fuchsin, Phenol, Ethanol, Methanol, Tween 80) |
100 m |
|
B |
Acid Alcohol ( Ethanol + Acid) |
100 m |
|
C |
Methylene Blue (alkaline, pH 10.7, KOH-adjusted) |
100 m |
Key Features:
-
Fully equivalent to GBL’s commercial product
-
Optimized for Ziehl–Neelsen hot staining protocol
-
Alkaline counterstain ensures sharp background contrast
-
Homogeneous smear coverage via surfactant addition
-
Shelf life up to 24 months under proper storage
Staining Results:
|
Observed Structure |
Appearance |
|---|---|
|
Acid-fast bacilli (AFB) |
Red |
|
Other microorganisms |
Blue |
|
Background |
Light blue |
Technical Documents
Related Posts
Related News
Technical Support and Product Consulting
You can use the form to find the most suitable chemical solution for your needs or to obtain detailed information about the technical specifications of our products.
Your requests are carefully reviewed and answered by Colin's expert team.
You can ask us about the product.
