1.0 M Tris-HCl (pH 8.0) is a high concentration buffer solution used in molecular biology and biochemistry experiments. Prepared using Tris (Tris(hydroxymethyl)aminomethane) and hydrochloric acid (HCl), this buffer is preferred to stabilize and keep the pH constant for reactions. It is an essential component for many biochemical applications such as DNA, RNA and protein studies.
Features
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pH Stabilization: Optimizes enzymatic and biochemical reactions by providing a stable environment at pH 8.0.
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High Concentration: Thanks to its 1.0 M concentration, it offers a wide range of uses by dilution.
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Precise Formulation: Provides reliable results in molecular biology and biochemistry studies.
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Stability: It can be stored for a long time and creates an effective buffer environment.
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Versatile Usage: It can be used in many applications from nucleic acid purification to protein analysis.
Areas of Use
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DNA and RNA studies:
It is used as a buffer solution in nucleic acid isolation, storage and analysis. -
Enzymatic Reactions:
It provides an ideal buffer for restriction enzymes, DNA polymerase and other enzymatic processes. -
Protein studies:
Optimizing the stability and activity of proteins -
Maintaining pH Balance:
It is a sensitive buffer solution for experiments requiring pH 8.0 in laboratory applications.
Storage Conditions
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Can be stored at room temperature (15-25°C) or in the refrigerator (2-8°C).
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Should be protected from direct sunlight and high humidity.
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Stored under sterile conditions to avoid contamination should be kept in a cool place.
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After use, the lid should be tightly closed and kept in a cool place.
Chemical and Biological Testing Solutions for Laboratories and R&D Centers
It is used as a buffer solution in DNA, RNA and protein studies.
Recommended Test Kits and Solutions for Universities and Educational Institutions
It is used as a buffer solution in biological and chemical experiments.
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